For the rotor gene q and other rotor gene cyclers we recommend using the rotor gene sybr green pcr kit which has been specially developed for fast cycling on these. Kits include kapa sybr fast qpcr master mix 2x which contains kapa sybr fast dna polymerase reaction buffer dntps sybr green i dye and mgcl 2 at a final concentration of 2 5 mm where noted master mixes contain instrument specific reference dyes while the universal kit includes rox high and rox low both 50x separately. Applied biosystems instruments assays and reagents have been trusted in the lab for over 20 years.
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